real time control implementation platform Search Results


simulink desktop real time  (MathWorks Inc)
94
MathWorks Inc simulink desktop real time
Simulink Desktop Real Time, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab simulink real time environment  (MathWorks Inc)
96
MathWorks Inc matlab simulink real time environment
Matlab Simulink Real Time Environment, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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q4 series hardware-in-the-loop board  (Quanser Consulting)
90
Quanser Consulting q4 series hardware-in-the-loop board
Q4 Series Hardware In The Loop Board, supplied by Quanser Consulting, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody goat anti rabbit immunoglobulin g igg h l horseradish peroxidase hrp  (Abcam)
99
Abcam antibody goat anti rabbit immunoglobulin g igg h l horseradish peroxidase hrp
Long non-coding RNAs (lncRNA) taurine upregulated gene 1 (TUG1) binds to miR-328-3p to upregulate sterol regulatory element binding protein 2 (SREBP-2) expression and enhance pancreatic β-cell viability and insulin secretion. (A) Upstream regulatory lncRNAs of miR-328-3p in human and mice predicted by the starBase database; the two circles in the figure represent the prediction results in human and mice respectively, and the central part represents the intersection of the two sets of data. (B) Binding sites between miR-328-3p and lncRNA TUG1 both in human and mice. (C) LncRNA TUG1 expression in the islet tissues of control and gestational diabetes mellitus (GDM) mice determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (D) Binding between miR-328-3p and lncRNA TUG1 confirmed by dual-luciferase reporter assay in islet cells transfected with miR-328-3p mimic or negative control (NC) mimic. (E) Binding of miR-328-3p and lncRNA TUG1 to the argonaute 2 (Ago2) antibody determined by RNA binding protein immunoprecipitation (RIP) assay. (F) Binding between miR-328-3p and lncRNA TUG1 determined by RNA pull-down assay; BETA-TC-6 cells were transfected with miR-328-3p mimic, overexpression (oe)-lncRNA TUG1 or both. (G) Expression of lncRNA TUG1, miR-328-3p and SREBP-2 in BETA-TC-6 cells determined by RT-qPCR. (H) BETA-TC-6 cell viability measured by cell counting kit-8 (CCK-8) assay. (I) Flow cytometric examination of BETA-TC-6 cell apoptosis. (J) Total insulin content in BETA-TC-6 cells measured by enzyme-linked immunosorbent assay (ELISA). (K) Insulin secretion in BETA-TC-6 cells measured by ELISA. IgG, immunoglobulin G. a P <0.05 vs. islet cells transfected with NC mimic or BETA-TC-6 cells transfected with oe-NC+NC mimic, b P <0.05 vs. BETA-TC-6 cells transfected with oe-NC+miR-328-3p mimic (n=10 for mice following each treatment). Cell experiments were repeated three times.
Antibody Goat Anti Rabbit Immunoglobulin G Igg H L Horseradish Peroxidase Hrp, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mex file  (MathWorks Inc)
90
MathWorks Inc mex file
Long non-coding RNAs (lncRNA) taurine upregulated gene 1 (TUG1) binds to miR-328-3p to upregulate sterol regulatory element binding protein 2 (SREBP-2) expression and enhance pancreatic β-cell viability and insulin secretion. (A) Upstream regulatory lncRNAs of miR-328-3p in human and mice predicted by the starBase database; the two circles in the figure represent the prediction results in human and mice respectively, and the central part represents the intersection of the two sets of data. (B) Binding sites between miR-328-3p and lncRNA TUG1 both in human and mice. (C) LncRNA TUG1 expression in the islet tissues of control and gestational diabetes mellitus (GDM) mice determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (D) Binding between miR-328-3p and lncRNA TUG1 confirmed by dual-luciferase reporter assay in islet cells transfected with miR-328-3p mimic or negative control (NC) mimic. (E) Binding of miR-328-3p and lncRNA TUG1 to the argonaute 2 (Ago2) antibody determined by RNA binding protein immunoprecipitation (RIP) assay. (F) Binding between miR-328-3p and lncRNA TUG1 determined by RNA pull-down assay; BETA-TC-6 cells were transfected with miR-328-3p mimic, overexpression (oe)-lncRNA TUG1 or both. (G) Expression of lncRNA TUG1, miR-328-3p and SREBP-2 in BETA-TC-6 cells determined by RT-qPCR. (H) BETA-TC-6 cell viability measured by cell counting kit-8 (CCK-8) assay. (I) Flow cytometric examination of BETA-TC-6 cell apoptosis. (J) Total insulin content in BETA-TC-6 cells measured by enzyme-linked immunosorbent assay (ELISA). (K) Insulin secretion in BETA-TC-6 cells measured by ELISA. IgG, immunoglobulin G. a P <0.05 vs. islet cells transfected with NC mimic or BETA-TC-6 cells transfected with oe-NC+NC mimic, b P <0.05 vs. BETA-TC-6 cells transfected with oe-NC+miR-328-3p mimic (n=10 for mice following each treatment). Cell experiments were repeated three times.
Mex File, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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virtex-4  (Xilinx Inc)
90
Xilinx Inc virtex-4
Long non-coding RNAs (lncRNA) taurine upregulated gene 1 (TUG1) binds to miR-328-3p to upregulate sterol regulatory element binding protein 2 (SREBP-2) expression and enhance pancreatic β-cell viability and insulin secretion. (A) Upstream regulatory lncRNAs of miR-328-3p in human and mice predicted by the starBase database; the two circles in the figure represent the prediction results in human and mice respectively, and the central part represents the intersection of the two sets of data. (B) Binding sites between miR-328-3p and lncRNA TUG1 both in human and mice. (C) LncRNA TUG1 expression in the islet tissues of control and gestational diabetes mellitus (GDM) mice determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (D) Binding between miR-328-3p and lncRNA TUG1 confirmed by dual-luciferase reporter assay in islet cells transfected with miR-328-3p mimic or negative control (NC) mimic. (E) Binding of miR-328-3p and lncRNA TUG1 to the argonaute 2 (Ago2) antibody determined by RNA binding protein immunoprecipitation (RIP) assay. (F) Binding between miR-328-3p and lncRNA TUG1 determined by RNA pull-down assay; BETA-TC-6 cells were transfected with miR-328-3p mimic, overexpression (oe)-lncRNA TUG1 or both. (G) Expression of lncRNA TUG1, miR-328-3p and SREBP-2 in BETA-TC-6 cells determined by RT-qPCR. (H) BETA-TC-6 cell viability measured by cell counting kit-8 (CCK-8) assay. (I) Flow cytometric examination of BETA-TC-6 cell apoptosis. (J) Total insulin content in BETA-TC-6 cells measured by enzyme-linked immunosorbent assay (ELISA). (K) Insulin secretion in BETA-TC-6 cells measured by ELISA. IgG, immunoglobulin G. a P <0.05 vs. islet cells transfected with NC mimic or BETA-TC-6 cells transfected with oe-NC+NC mimic, b P <0.05 vs. BETA-TC-6 cells transfected with oe-NC+miR-328-3p mimic (n=10 for mice following each treatment). Cell experiments were repeated three times.
Virtex 4, supplied by Xilinx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab/simulink simpowersystem platform  (MathWorks Inc)
90
MathWorks Inc matlab/simulink simpowersystem platform
Long non-coding RNAs (lncRNA) taurine upregulated gene 1 (TUG1) binds to miR-328-3p to upregulate sterol regulatory element binding protein 2 (SREBP-2) expression and enhance pancreatic β-cell viability and insulin secretion. (A) Upstream regulatory lncRNAs of miR-328-3p in human and mice predicted by the starBase database; the two circles in the figure represent the prediction results in human and mice respectively, and the central part represents the intersection of the two sets of data. (B) Binding sites between miR-328-3p and lncRNA TUG1 both in human and mice. (C) LncRNA TUG1 expression in the islet tissues of control and gestational diabetes mellitus (GDM) mice determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (D) Binding between miR-328-3p and lncRNA TUG1 confirmed by dual-luciferase reporter assay in islet cells transfected with miR-328-3p mimic or negative control (NC) mimic. (E) Binding of miR-328-3p and lncRNA TUG1 to the argonaute 2 (Ago2) antibody determined by RNA binding protein immunoprecipitation (RIP) assay. (F) Binding between miR-328-3p and lncRNA TUG1 determined by RNA pull-down assay; BETA-TC-6 cells were transfected with miR-328-3p mimic, overexpression (oe)-lncRNA TUG1 or both. (G) Expression of lncRNA TUG1, miR-328-3p and SREBP-2 in BETA-TC-6 cells determined by RT-qPCR. (H) BETA-TC-6 cell viability measured by cell counting kit-8 (CCK-8) assay. (I) Flow cytometric examination of BETA-TC-6 cell apoptosis. (J) Total insulin content in BETA-TC-6 cells measured by enzyme-linked immunosorbent assay (ELISA). (K) Insulin secretion in BETA-TC-6 cells measured by ELISA. IgG, immunoglobulin G. a P <0.05 vs. islet cells transfected with NC mimic or BETA-TC-6 cells transfected with oe-NC+NC mimic, b P <0.05 vs. BETA-TC-6 cells transfected with oe-NC+miR-328-3p mimic (n=10 for mice following each treatment). Cell experiments were repeated three times.
Matlab/Simulink Simpowersystem Platform, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rthawk real-time imaging platform  (HeartVista)
90
HeartVista rthawk real-time imaging platform
Long non-coding RNAs (lncRNA) taurine upregulated gene 1 (TUG1) binds to miR-328-3p to upregulate sterol regulatory element binding protein 2 (SREBP-2) expression and enhance pancreatic β-cell viability and insulin secretion. (A) Upstream regulatory lncRNAs of miR-328-3p in human and mice predicted by the starBase database; the two circles in the figure represent the prediction results in human and mice respectively, and the central part represents the intersection of the two sets of data. (B) Binding sites between miR-328-3p and lncRNA TUG1 both in human and mice. (C) LncRNA TUG1 expression in the islet tissues of control and gestational diabetes mellitus (GDM) mice determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (D) Binding between miR-328-3p and lncRNA TUG1 confirmed by dual-luciferase reporter assay in islet cells transfected with miR-328-3p mimic or negative control (NC) mimic. (E) Binding of miR-328-3p and lncRNA TUG1 to the argonaute 2 (Ago2) antibody determined by RNA binding protein immunoprecipitation (RIP) assay. (F) Binding between miR-328-3p and lncRNA TUG1 determined by RNA pull-down assay; BETA-TC-6 cells were transfected with miR-328-3p mimic, overexpression (oe)-lncRNA TUG1 or both. (G) Expression of lncRNA TUG1, miR-328-3p and SREBP-2 in BETA-TC-6 cells determined by RT-qPCR. (H) BETA-TC-6 cell viability measured by cell counting kit-8 (CCK-8) assay. (I) Flow cytometric examination of BETA-TC-6 cell apoptosis. (J) Total insulin content in BETA-TC-6 cells measured by enzyme-linked immunosorbent assay (ELISA). (K) Insulin secretion in BETA-TC-6 cells measured by ELISA. IgG, immunoglobulin G. a P <0.05 vs. islet cells transfected with NC mimic or BETA-TC-6 cells transfected with oe-NC+NC mimic, b P <0.05 vs. BETA-TC-6 cells transfected with oe-NC+miR-328-3p mimic (n=10 for mice following each treatment). Cell experiments were repeated three times.
Rthawk Real Time Imaging Platform, supplied by HeartVista, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab/simulink diagram  (MathWorks Inc)
96
MathWorks Inc matlab/simulink diagram
Long non-coding RNAs (lncRNA) taurine upregulated gene 1 (TUG1) binds to miR-328-3p to upregulate sterol regulatory element binding protein 2 (SREBP-2) expression and enhance pancreatic β-cell viability and insulin secretion. (A) Upstream regulatory lncRNAs of miR-328-3p in human and mice predicted by the starBase database; the two circles in the figure represent the prediction results in human and mice respectively, and the central part represents the intersection of the two sets of data. (B) Binding sites between miR-328-3p and lncRNA TUG1 both in human and mice. (C) LncRNA TUG1 expression in the islet tissues of control and gestational diabetes mellitus (GDM) mice determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (D) Binding between miR-328-3p and lncRNA TUG1 confirmed by dual-luciferase reporter assay in islet cells transfected with miR-328-3p mimic or negative control (NC) mimic. (E) Binding of miR-328-3p and lncRNA TUG1 to the argonaute 2 (Ago2) antibody determined by RNA binding protein immunoprecipitation (RIP) assay. (F) Binding between miR-328-3p and lncRNA TUG1 determined by RNA pull-down assay; BETA-TC-6 cells were transfected with miR-328-3p mimic, overexpression (oe)-lncRNA TUG1 or both. (G) Expression of lncRNA TUG1, miR-328-3p and SREBP-2 in BETA-TC-6 cells determined by RT-qPCR. (H) BETA-TC-6 cell viability measured by cell counting kit-8 (CCK-8) assay. (I) Flow cytometric examination of BETA-TC-6 cell apoptosis. (J) Total insulin content in BETA-TC-6 cells measured by enzyme-linked immunosorbent assay (ELISA). (K) Insulin secretion in BETA-TC-6 cells measured by ELISA. IgG, immunoglobulin G. a P <0.05 vs. islet cells transfected with NC mimic or BETA-TC-6 cells transfected with oe-NC+NC mimic, b P <0.05 vs. BETA-TC-6 cells transfected with oe-NC+miR-328-3p mimic (n=10 for mice following each treatment). Cell experiments were repeated three times.
Matlab/Simulink Diagram, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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real time windows target software  (MathWorks Inc)
90
MathWorks Inc real time windows target software
Long non-coding RNAs (lncRNA) taurine upregulated gene 1 (TUG1) binds to miR-328-3p to upregulate sterol regulatory element binding protein 2 (SREBP-2) expression and enhance pancreatic β-cell viability and insulin secretion. (A) Upstream regulatory lncRNAs of miR-328-3p in human and mice predicted by the starBase database; the two circles in the figure represent the prediction results in human and mice respectively, and the central part represents the intersection of the two sets of data. (B) Binding sites between miR-328-3p and lncRNA TUG1 both in human and mice. (C) LncRNA TUG1 expression in the islet tissues of control and gestational diabetes mellitus (GDM) mice determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (D) Binding between miR-328-3p and lncRNA TUG1 confirmed by dual-luciferase reporter assay in islet cells transfected with miR-328-3p mimic or negative control (NC) mimic. (E) Binding of miR-328-3p and lncRNA TUG1 to the argonaute 2 (Ago2) antibody determined by RNA binding protein immunoprecipitation (RIP) assay. (F) Binding between miR-328-3p and lncRNA TUG1 determined by RNA pull-down assay; BETA-TC-6 cells were transfected with miR-328-3p mimic, overexpression (oe)-lncRNA TUG1 or both. (G) Expression of lncRNA TUG1, miR-328-3p and SREBP-2 in BETA-TC-6 cells determined by RT-qPCR. (H) BETA-TC-6 cell viability measured by cell counting kit-8 (CCK-8) assay. (I) Flow cytometric examination of BETA-TC-6 cell apoptosis. (J) Total insulin content in BETA-TC-6 cells measured by enzyme-linked immunosorbent assay (ELISA). (K) Insulin secretion in BETA-TC-6 cells measured by ELISA. IgG, immunoglobulin G. a P <0.05 vs. islet cells transfected with NC mimic or BETA-TC-6 cells transfected with oe-NC+NC mimic, b P <0.05 vs. BETA-TC-6 cells transfected with oe-NC+miR-328-3p mimic (n=10 for mice following each treatment). Cell experiments were repeated three times.
Real Time Windows Target Software, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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system generator (xsg)  (Xilinx Inc)
90
Xilinx Inc system generator (xsg)
Long non-coding RNAs (lncRNA) taurine upregulated gene 1 (TUG1) binds to miR-328-3p to upregulate sterol regulatory element binding protein 2 (SREBP-2) expression and enhance pancreatic β-cell viability and insulin secretion. (A) Upstream regulatory lncRNAs of miR-328-3p in human and mice predicted by the starBase database; the two circles in the figure represent the prediction results in human and mice respectively, and the central part represents the intersection of the two sets of data. (B) Binding sites between miR-328-3p and lncRNA TUG1 both in human and mice. (C) LncRNA TUG1 expression in the islet tissues of control and gestational diabetes mellitus (GDM) mice determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (D) Binding between miR-328-3p and lncRNA TUG1 confirmed by dual-luciferase reporter assay in islet cells transfected with miR-328-3p mimic or negative control (NC) mimic. (E) Binding of miR-328-3p and lncRNA TUG1 to the argonaute 2 (Ago2) antibody determined by RNA binding protein immunoprecipitation (RIP) assay. (F) Binding between miR-328-3p and lncRNA TUG1 determined by RNA pull-down assay; BETA-TC-6 cells were transfected with miR-328-3p mimic, overexpression (oe)-lncRNA TUG1 or both. (G) Expression of lncRNA TUG1, miR-328-3p and SREBP-2 in BETA-TC-6 cells determined by RT-qPCR. (H) BETA-TC-6 cell viability measured by cell counting kit-8 (CCK-8) assay. (I) Flow cytometric examination of BETA-TC-6 cell apoptosis. (J) Total insulin content in BETA-TC-6 cells measured by enzyme-linked immunosorbent assay (ELISA). (K) Insulin secretion in BETA-TC-6 cells measured by ELISA. IgG, immunoglobulin G. a P <0.05 vs. islet cells transfected with NC mimic or BETA-TC-6 cells transfected with oe-NC+NC mimic, b P <0.05 vs. BETA-TC-6 cells transfected with oe-NC+miR-328-3p mimic (n=10 for mice following each treatment). Cell experiments were repeated three times.
System Generator (Xsg), supplied by Xilinx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab/simulink  (MathWorks Inc)
90
MathWorks Inc matlab/simulink
Long non-coding RNAs (lncRNA) taurine upregulated gene 1 (TUG1) binds to miR-328-3p to upregulate sterol regulatory element binding protein 2 (SREBP-2) expression and enhance pancreatic β-cell viability and insulin secretion. (A) Upstream regulatory lncRNAs of miR-328-3p in human and mice predicted by the starBase database; the two circles in the figure represent the prediction results in human and mice respectively, and the central part represents the intersection of the two sets of data. (B) Binding sites between miR-328-3p and lncRNA TUG1 both in human and mice. (C) LncRNA TUG1 expression in the islet tissues of control and gestational diabetes mellitus (GDM) mice determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (D) Binding between miR-328-3p and lncRNA TUG1 confirmed by dual-luciferase reporter assay in islet cells transfected with miR-328-3p mimic or negative control (NC) mimic. (E) Binding of miR-328-3p and lncRNA TUG1 to the argonaute 2 (Ago2) antibody determined by RNA binding protein immunoprecipitation (RIP) assay. (F) Binding between miR-328-3p and lncRNA TUG1 determined by RNA pull-down assay; BETA-TC-6 cells were transfected with miR-328-3p mimic, overexpression (oe)-lncRNA TUG1 or both. (G) Expression of lncRNA TUG1, miR-328-3p and SREBP-2 in BETA-TC-6 cells determined by RT-qPCR. (H) BETA-TC-6 cell viability measured by cell counting kit-8 (CCK-8) assay. (I) Flow cytometric examination of BETA-TC-6 cell apoptosis. (J) Total insulin content in BETA-TC-6 cells measured by enzyme-linked immunosorbent assay (ELISA). (K) Insulin secretion in BETA-TC-6 cells measured by ELISA. IgG, immunoglobulin G. a P <0.05 vs. islet cells transfected with NC mimic or BETA-TC-6 cells transfected with oe-NC+NC mimic, b P <0.05 vs. BETA-TC-6 cells transfected with oe-NC+miR-328-3p mimic (n=10 for mice following each treatment). Cell experiments were repeated three times.
Matlab/Simulink, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Long non-coding RNAs (lncRNA) taurine upregulated gene 1 (TUG1) binds to miR-328-3p to upregulate sterol regulatory element binding protein 2 (SREBP-2) expression and enhance pancreatic β-cell viability and insulin secretion. (A) Upstream regulatory lncRNAs of miR-328-3p in human and mice predicted by the starBase database; the two circles in the figure represent the prediction results in human and mice respectively, and the central part represents the intersection of the two sets of data. (B) Binding sites between miR-328-3p and lncRNA TUG1 both in human and mice. (C) LncRNA TUG1 expression in the islet tissues of control and gestational diabetes mellitus (GDM) mice determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (D) Binding between miR-328-3p and lncRNA TUG1 confirmed by dual-luciferase reporter assay in islet cells transfected with miR-328-3p mimic or negative control (NC) mimic. (E) Binding of miR-328-3p and lncRNA TUG1 to the argonaute 2 (Ago2) antibody determined by RNA binding protein immunoprecipitation (RIP) assay. (F) Binding between miR-328-3p and lncRNA TUG1 determined by RNA pull-down assay; BETA-TC-6 cells were transfected with miR-328-3p mimic, overexpression (oe)-lncRNA TUG1 or both. (G) Expression of lncRNA TUG1, miR-328-3p and SREBP-2 in BETA-TC-6 cells determined by RT-qPCR. (H) BETA-TC-6 cell viability measured by cell counting kit-8 (CCK-8) assay. (I) Flow cytometric examination of BETA-TC-6 cell apoptosis. (J) Total insulin content in BETA-TC-6 cells measured by enzyme-linked immunosorbent assay (ELISA). (K) Insulin secretion in BETA-TC-6 cells measured by ELISA. IgG, immunoglobulin G. a P <0.05 vs. islet cells transfected with NC mimic or BETA-TC-6 cells transfected with oe-NC+NC mimic, b P <0.05 vs. BETA-TC-6 cells transfected with oe-NC+miR-328-3p mimic (n=10 for mice following each treatment). Cell experiments were repeated three times.

Journal: Diabetes & Metabolism Journal

Article Title: Long Non-Coding RNA TUG1 Attenuates Insulin Resistance in Mice with Gestational Diabetes Mellitus via Regulation of the MicroRNA-328-3p/SREBP-2/ERK Axis

doi: 10.4093/dmj.2021.0216

Figure Lengend Snippet: Long non-coding RNAs (lncRNA) taurine upregulated gene 1 (TUG1) binds to miR-328-3p to upregulate sterol regulatory element binding protein 2 (SREBP-2) expression and enhance pancreatic β-cell viability and insulin secretion. (A) Upstream regulatory lncRNAs of miR-328-3p in human and mice predicted by the starBase database; the two circles in the figure represent the prediction results in human and mice respectively, and the central part represents the intersection of the two sets of data. (B) Binding sites between miR-328-3p and lncRNA TUG1 both in human and mice. (C) LncRNA TUG1 expression in the islet tissues of control and gestational diabetes mellitus (GDM) mice determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (D) Binding between miR-328-3p and lncRNA TUG1 confirmed by dual-luciferase reporter assay in islet cells transfected with miR-328-3p mimic or negative control (NC) mimic. (E) Binding of miR-328-3p and lncRNA TUG1 to the argonaute 2 (Ago2) antibody determined by RNA binding protein immunoprecipitation (RIP) assay. (F) Binding between miR-328-3p and lncRNA TUG1 determined by RNA pull-down assay; BETA-TC-6 cells were transfected with miR-328-3p mimic, overexpression (oe)-lncRNA TUG1 or both. (G) Expression of lncRNA TUG1, miR-328-3p and SREBP-2 in BETA-TC-6 cells determined by RT-qPCR. (H) BETA-TC-6 cell viability measured by cell counting kit-8 (CCK-8) assay. (I) Flow cytometric examination of BETA-TC-6 cell apoptosis. (J) Total insulin content in BETA-TC-6 cells measured by enzyme-linked immunosorbent assay (ELISA). (K) Insulin secretion in BETA-TC-6 cells measured by ELISA. IgG, immunoglobulin G. a P <0.05 vs. islet cells transfected with NC mimic or BETA-TC-6 cells transfected with oe-NC+NC mimic, b P <0.05 vs. BETA-TC-6 cells transfected with oe-NC+miR-328-3p mimic (n=10 for mice following each treatment). Cell experiments were repeated three times.

Article Snippet: After rewarming for 30 minutes, another incubation was implemented with addition of secondary antibody goat anti-rabbit immunoglobulin G (IgG) H&L horseradish peroxidase (HRP) (ab6721, 1:200, Abcam) at ambient temperature for 1 hour.

Techniques: Binding Assay, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Luciferase, Reporter Assay, Transfection, Negative Control, RNA Binding Assay, Immunoprecipitation, Pull Down Assay, Over Expression, Cell Counting, CCK-8 Assay, Enzyme-linked Immunosorbent Assay